Downsampling cells from each group for IDER-based similarity calculation.
Usage
downsampling(
metadata,
n.size = 35,
seed = 12345,
include = FALSE,
replace = FALSE,
lower.cutoff = 3
)Arguments
- metadata
Data frame. It includes at least 2 columns, label and batch. Each row corresponds to one cell. Required.
- n.size
Numeric. The number of cells used in each group. (Default: 35)
- seed
Numeric. Seed used to sample. (Default: 12345)
- include
Boolean. Using `include = TRUE` to include the group smaller than required size. (Default: FALSE)
- replace
Boolean. Using `replace = TRUE` if the group is smaller than required size and some cells will be repeatedly used. (Default: FALSE)
- lower.cutoff
Numeric. The minimum size of groups to keep. (Default: 3)